have been applied to enhance astaxanthin output in Ast010 and Ast017, which possibly implied that the specific action of CrtZ and CrtW* is lower. Too much overexpression of heterologous genes can lead to a metabolic stress or protein misfolding, as well as non particular side reactions [24].

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CrtW converts β-carotene into keto-carotenoids, that have a keto team on the 4, four ' placement with the ionone ring, and it can be utilized to synthesize canthaxanthin, which consequently could be converted into astaxanthin by CrtZ. The canthaxanthin making strain Can003 was made by inserting a crtYW*

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web-site, had a great deal more lycopene accumulation then that of CAR026, even there wasn’t any modification on lycopene biosynthesis pathway [fourteen]. A large amount of lycopene accumulation in Ast004 was because of the insufficient activity of CrtY. Appropriately, added copies of crtY

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